Data/hora: 11/06/2024 00:48:54

Provedor de dados: 	Brazilian Journal of Microbiology	
País: 	Brazil	
Título: 	Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene	
Autores: 	Lazim,Zainab Mat; Hadibarata,Tony.	
Data: 	2016-09-01	
Ano: 	2016	
Palavras-chave: 	Metabolites; Ligninolytic enzymes; Polyporus sp. S133; Non-ionic surfactants; Solubilization.	
Resumo: 	ABSTRACT This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV-vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the culture medium confirmed the biotransformation of fluorene. Two metabolites, 9-fluorenol (λmax 270, tR 8.0 min and m/z 254) and protocatechuic acid (λmax 260, tR 11.3 min and m/z 370), were identified in the treated medium.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300610	
Editor: 	Sociedade Brasileira de Microbiologia	
Relação: 	10.1016/j.bjm.2016.04.015	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Microbiology  v.47 n.3 2016	
Fonte: 	info:eu-repo/semantics/openAccess