Data/hora: 01/06/2024 02:50:35

Provedor de dados: | Revista de Microbiologia
País: | Brazil
Título: | Production and action pattern of inulinase from Aspergillus Niger-245: hydrolysis of inulin from several sources
Autores: | Cruz,Vinícius D’Arcadia; Belote,Juliana Gisele; Belline,Márcia Zilioli; Cruz,Rubens.
Data: | 1998-10-01
Ano: | 1998
Palavras-chave: | Inulinase; Aspergillus niger; Fructose syrup; Inulin.
Resumo: | A strain of Aspergillus niger isolated from soil samples showed great capacity to produce extracellular inulinase. Although the enzyme has been synthesized in presence of monosaccharides, sucrose and sugar cane molasse, the productivity was significantly higher (p&amp;lt;0.05) when the microorganism was inoculated in media formulated with dahlia extract and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with casein and other sources of proteic nitrogen, comparatively to the mineral nitrogen. However, statistic significance (p&amp;lt;0.01) only was found between the productivity obtained in the medium prepared with casein and ammonium sulphate. The optimum pH of the purified enzyme for inulin hydrolysis was found between 4.0 and 4.5 and the optimun temperature at 60oC. When treated by 30 minutes in this temperature no loss of activity was observed. The enzyme showed capacity to hydrolyse sucrose, raffinose and inulin from which it liberated only fructose units showing, therefore, an exo-action mechanism. Acting on inulins from several sources, the enzyme showed larger hydrolysis speed on the polissaccharide from chicory (Cichorium intibus), comparatively, to the inulins from dahlia (Dahlia pinnata) and Jerusalem artichoke (Helianthus tuberosus) roots.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400013
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141998000400013
Formato: | text/html
Fonte: | Revista de Microbiologia  v.29 n.4 1998
Fonte: | info:eu-repo/semantics/openAccess