Data/hora: 09/06/2024 11:54:13

Provedor de dados: | 59
País: | Brazil
Título: | Discrimination of members of the Mycobacterium avium complex by polymerase chain reaction
Autores: | Sircili,Marcelo Palma; Roxo,Eliana; Leão,Sylvia Cardoso.
Data: | 1999-04-01
Ano: | 1999
Palavras-chave: | Amplification; PCR; MAC; Mycobacterium; Identification.
Resumo: | Mycobacterium avium complex (MAC) species cannot be discriminated by the usual methods of biochemical identification of mycobacteria. This study showed that amplification by PCR of DT1 and DT6, two single copy sequences identified in the genome of M. avium serotype 2, the insertion sequence IS1245, found to be consistently present in M. avium strains and the heat-shock protein gene hsp65, followed by restriction polymorphism analysis, are rapid and accurate tests for the differentiation of the species M. avium, M. intracellulare, and M. scrofulaceum.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200011
Editor: | Sociedade Brasileira de Microbiologia
Relação: | 10.1590/S0001-37141999000200011
Formato: | text/html
Fonte: | Revista de Microbiologia  v.30 n.2 1999
Fonte: | info:eu-repo/semantics/openAccess