Data/hora: 16/06/2024 11:10:48

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Polymerase chain reaction and blood culture in blood donors screened by ELISA test for Chagas' disease	
Autores: 	Kinoshita-Yanaga,Andréa Tieko; Bértoli,Marta; Martins,Vanessa Aparecida; Mizutani,Angélica Sayuri; Toledo,Max Jean de Ornelas; Araújo,Silvana Marques de; Gomes,Mônica Lúcia.	
Data: 	2011-03-01	
Ano: 	2011	
Palavras-chave: 	Trypanosoma cruzi/detection; ELISA/use for screening; Blood donors; Blood culture; PCR; Blood bank/screened donors.	
Resumo: 	The objective of this study was to evaluate, through blood culture and PCR, the results of the ELISA for Chagas' disease in the screening of blood donors in the public blood-supply network of the state of Paraná, Brazil, and to map the epidemiological profile of the donors with respect to their risk of infection by Trypanosoma cruzi. The negative and positive results of the ELISA were confirmed by blood culture and PCR for 190/191 individuals (99.5%). For one individual (0.5%), the ELISA was inconclusive, blood culture and IIF were negative, and IHA and PCR positive. Three individuals (1.6%) were positive for T. cruzi on all the tests. Donors were predominantly female, and natives of Paraná, of rural origin, had observed or been informed of the presence of the vector in the municipalities where they resided, had never received a blood transfusion, had donated blood 1 to 4 times, and reported no cases of Chagas' disease in their families. We concluded that PCR and blood culturing have excellent potential for confirming the results of the ELISA, and that candidate blood donors with negative or positive tests have a similar risk of infection by T. cruzi, indicating that the ELISA test is sufficiently safe for screening blood prior to use.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000100007	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502011000100007	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.47 n.1 2011	
Fonte: 	info:eu-repo/semantics/openAccess