Data/hora: 16/06/2024 14:57:43

Provedor de dados: | Brazilian Journal of Pharmaceutical Sciences
País: | Brazil
Título: | Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium
Autores: | Trevisan,Daliah Alves Coelho; Silva,Alex Fiori da; Negri,Melyssa; Abreu Filho,Benício Alves de; Machinski Junior,Miguel; Patussi,Eliana Valéria; Campanerut-Sá,Paula Aline Zanetti; Mikcha,Jane Martha Graton.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Carvacrol; Biofilm; Antibacterial activity; Salmonella Typhimurium.
Resumo: | ABSTRACT The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and the time-kill curve and scanning electron microscopy (SEM) were performed to evaluate antibacterial activity. Antibiofilm activity was evaluated by quantifying total biomass using crystal violet assay, and metabolic activity was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The action of carvacrol against preformed biofilm on polypropylene and stainless steel was also evaluated by colony counting and SEM. The MIC and MBC was 312 µg mL-1. Carvacrol at MIC and 2 x MIC eliminated cells after 6 and 1 h of treatment, respectively, as exhibited in the time-kill curve. The greatest reduction in biofilm biomass and metabolic activity was 1,719 OD550 and 0,089 OD550 respectively, both at 4 x MIC of carvacrol. In carvacrol treated biofilms of S. Typhimurium on polypropylene, a reduction of 5.12 log was observed with 4 x MIC, while on stainless steel, carvacrol at 4 x MIC reduced bacterial counts by 5 log. The results showed that carvacrol exhibits antibacterial activity and can be used as an alternative for the control of S. Typhimurium biofilms.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100612
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s2175-97902018000117229
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.54 n.1 2018
Fonte: | info:eu-repo/semantics/openAccess