Data/hora: 08/06/2024 10:23:27

Provedor de dados: | 60
País: | Brazil
Título: | Production of intravenous human dengue immunoglobulin from Brazilian-blood donors
Autores: | Gouveia,Frederico Leite; Oliveira,Renata Mendes Batista de; Lucena,Antônio Edson de Souza; Cordeiro,Marli Tenório; Leite,Ana Cristina Lima.
Data: | 2013-12-01
Ano: | 2013
Palavras-chave: | Viral infections; Dengue/treatment; Dengue/virus; Dengue/neutralization; Immunoglobulins/preparation; Plasma fractionation; Virus neutralization.
Resumo: | Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG collected from American blood donations was found to have low anti-dengue antibody titers. Overall, this anti-DENV IgG represents an important step in the study of the therapeutic potential and safety of a specific antibody that neutralizes the dengue virus in humans.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400022
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502013000400022
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.49 n.4 2013
Fonte: | info:eu-repo/semantics/openAccess