Data/hora: 08/06/2024 13:51:01

Provedor de dados: | 60
País: | Brazil
Título: | L-arginine, a nitric oxide precursor, reduces dapsone-induced methemoglobinemia in rats
Autores: | Moraes,Natália Valadares de; Bergamaschi,Mateus Machado; Bianchi,Maria de Lourdes Pires; Bragheto,Juliana Bordinassi; Malfará,Wilson Roberto; Queiroz,Regina Helena Costa.
Data: | 2012-03-01
Ano: | 2012
Palavras-chave: | Dapsone; Methemoglobinemia; L-arginine; Nitric oxide; L-NAME.
Resumo: | Dapsone use is frequently associated to hematological side effects such as methemoglobinemia and hemolytic anemia, which are related to N-hydroxylation mediated by the P450 enzyme system. The aim of the present study was to evaluate the influence of L-arginine supplementation, a precursor for the synthesis of nitric oxide, as single or multiple dose regimens on dapsone-induced methemoglobinemia. Male Wistar rats were treated with L-arginine at 5, 15, 30, 60 and 180 mg/kg doses (p.o., gavage) in single or multiple dose regimens 2 hours prior to dapsone administration (40 mg/kg, i.p.). The effect of the nitric oxide synthase inhibitor L-NAME was investigated by treatment with multiple doses of 30 mg/kg (p.o., gavage) 2 hours before dapsone administration. Blood samples were collected 2 hours after dapsone administration. Erythrocytic methemoglobin levels were assayed by spectrophotometry. The results showed that multiple dose supplementations with 5 and 15 mg/kg L-arginine reduced dapsone-induced methemoglobin levels. This effect is mediated by nitric oxide formation, since the reduction in methemoglobin levels by L-arginine is blocked by simultaneous administration with L-NAME, a nitric oxide synthase inhibitor.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000100010
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502012000100010
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.48 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess