Data/hora: 03/06/2024 19:07:09

Provedor de dados: | 60
País: | Brazil
Título: | Salacia campestris root bark extract: peroxidase inhibition, antioxidant and antiradical profile
Autores: | Vellosa,José Carlos Rebuglio; Khalil,Najeh Maissar; Gutierres,Vânia Ortega; Santos,Vânia Aparecida de Freitas Formenton Macedo dos; Furlan,Maysa; Brunetti,Iguatemy Lourenço; Oliveira,Olga Maria Mascarenhas de Faria.
Data: | 2009-03-01
Ano: | 2009
Palavras-chave: | Salacia campestris Walp/antioxidant properties; Hippocrateaceae; Myeloperoxidase; Reactive oxygen species; Antioxidants; Free radicals.
Resumo: | Reactive oxygen species (ROS) and free radical species have been implicated in initiating or accompanying many diseases in living organisms; there is thus, a continual need for antioxidants molecules to inactivate ROS/free radicals. Many studies of plants crude extracts have demonstrated free-radical scavenging and antioxidant action. Salacia species have long been used, in several countries, as traditional medicines against certain diseases and for their anti-inflammatory properties. In this study, Salacia campestris Walp (Hippocrateaceae) root bark ethanol extract (ScEtOH) was assessed for its ability to scavenge free radicals and reactive oxygen species; the results were expressed as percentage inhibition of the active species. ScEtOH was efficient against studied species: DPPH radical (obtained inhibition = 30%), ABTS•+ (IC50 = 1.8±0.8 μg/mL), HOCl (IC50 = 1.7 ± 0.1 μg/mL), O2•- (obtained inhibition = 32%), and NO• (obtained inhibition = 18 %). Peroxidase activity inhibition was evaluated through the guaiacol oxidation reaction catalyzed by hemin, HRP and myeloperoxidase (MPO); data showed that ScEtOH at 10 μg/mL led to 54 and 51% of inhibition, respectively, for the hemin and HRP systems. In the MPO system, ScEtOH promoted a 50% inhibition at 8.9 μg/mL, whereas quercetin, a powerful MPO inhibitor, inhibited this system at 1.35 μg/mL.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100012
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502009000100012
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.45 n.1 2009
Fonte: | info:eu-repo/semantics/openAccess