Data/hora: 04/06/2024 08:52:21

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination	
Autores: 	Razzaq,Syed Naeem; Ashfaq,Muhammad; Khan,Islam Ullah; Mariam,Irfana; Razzaq,Syed Saleem; Mustafa,Ghulam; Zubair,Muhammad.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Gatifloxacin; Dexamethasone; High performance liquid chromatography; Validation; Plasma; Degradation products.	
Resumo: 	Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100607	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s2175-97902017000115177	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.53 n.1 2017	
Fonte: 	info:eu-repo/semantics/openAccess