Data/hora: 04/06/2024 02:20:28

Provedor de dados: | 60
País: | Brazil
Título: | Importance of the agar-media in the evaluation of bacteriocin activity against the same test-microorganisms
Autores: | Azevedo,Pamela Oliveira de Souza de; Molinari,Francesco; Oliveira,Ricardo Pinheiro de Souza.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Bacteriocin; Growth inhibition; Solid medium; Agar well diffusion assay.
Resumo: | Abstract Bacteriocins are peptides produced by various species of bacteria, especially lactic acid bacteria, which exhibit a large spectrum of action against spoilage bacteria and foodborne pathogens. Successful application of techniques for quantitative or qualitative bacteriocin determination relies not only on the sensitivity of the test-microorganisms, but also on the agar-medium employed. Cell free supernatants are routinely used to preliminary screen for antimicrobial activity of bacteria by means of the agar well diffusion method, but the supernatant may also include other molecules (such as medium components and/or intracellular compounds) accidentally released during cell free supernatant preparation, which may interfere with the assay. Reproducibility of bacteriocin activity against the same test-microorganisms is an important factor to be considered. Unfortunately, no specific information about bioassays standardization to determine bacteriocin activity is available in the literature. In this work, growth inhibition by means of the agar well diffusion assays were carried out on different agar-media showing a strong dependence on the agar-medium used, indicating that the inhibitory effects could also depend on the diffusion of exudates that are included in the cell-free supernatant. The results presented in this communication show that selection of the agar-medium is crucial for the bioassay response.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100301
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s2175-97902018000117533
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.54 n.1 2018
Fonte: | info:eu-repo/semantics/openAccess