Data/hora: 04/06/2024 13:28:10

Provedor de dados: 	Brazilian Journal of Pharmaceutical Sciences	
País: 	Brazil	
Título: 	Simultaneous detection of three antineoplastic drugs on gloves by liquid chromatography with diode array detector	
Autores: 	Alcântara,Adélia Maria Pimenta de Pádua; Vitor,Ricardo Vilela; Vieira,Elisabeth Pizzamiglio; Martins,Isarita.	
Data: 	2010-12-01	
Ano: 	2010	
Palavras-chave: 	Antineoplastic drugs/detection; Gloves/toxicologial analysis; High Performance Liquid Chromatography/toxicologial analysis; Occupational exposure.	
Resumo: 	The aim of this study was to develop a method for simultaneous detection of antineoplastic drugs on gloves since, in occupational exposure, the main contamination route is through dermal contact, which may occur via prolonged contact with contaminated surfaces or materials. The assay was performed by liquid chromatography using the following conditions for the detection of 5-fluorouracil (5-FU), methotrexate (MTX) and paclitaxel (TAX): diode array detection and UV quantification at 195 nm for TAX, at 265 nm for 5-FU and at 302 nm for MTX; ODS column (250 x 4 mm, 5 μm) with a similar guard column; mobile phase consisted of water (pH 4)-methanol-acetonitrile (35:15:50, v/v/v) with a flow rate of 1 mL min-1. The method presented a linear range from 0.25 to 20 μg mL-1 with r² higher than 0.99. Repeatability was &lt;15% and satisfactory extraction efficiency was obtained when liquid-liquid extraction with ethyl acetate was used for 5-FU and TAX. Satisfactory solid phase extraction was also achieved with C18 cartridges and elution with methanol for MTX. The diode array detector allowed drug quantification at a concentration &amp;gt; 0.25 μg mL-1 in samples, although detection was possible in samples that presented values of around 0.1 μg mL-1. The results obtained suggest that the method developed can be applied for the simultaneous determination of the drugs studied and can be considered useful in exposure assessment for health care workers.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000400015	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502010000400015	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.46 n.4 2010	
Fonte: 	info:eu-repo/semantics/openAccess