Data/hora: 09/06/2024 10:43:48

Provedor de dados: | Brazilian Journal of Pharmaceutical Sciences
País: | Brazil
Título: | Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
Autores: | Emir,Ahmet; Emir,Ceren; Bozkurt,Buket; Onur,Mustafa Ali; Somer,Nehir Unver; Kaya,Gulen Irem.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Alkaloids; Amaryllidaceae; Plant extracts; High Performance Liquid Chromatography/method validation; High Perfomance Liquid Chromatography/quantitative analysis.
Resumo: | ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100603
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/s2175-97902017000115063
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.53 n.1 2017
Fonte: | info:eu-repo/semantics/openAccess