Data/hora: 04/06/2024 05:21:38

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles	
Autores: 	Mattos,Ana Cristina de; Khalil,Najeh Maissar; Mainardes,Rubiana Mara.	
Data: 	2013-03-01	
Ano: 	2013	
Palavras-chave: 	Fluorouracil/determination; Nanoparticles; High performance liquid chromatography/quantitative analysis; High performance liquid chromatography/method validation.	
Resumo: 	The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000100013	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502013000100013	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.49 n.1 2013	
Fonte: 	info:eu-repo/semantics/openAccess