Data/hora: 04/06/2024 10:18:51

Provedor de dados: | 60
País: | Brazil
Título: | Adhesion and biofilm formation by Staphylococcus aureus from food processing plants as affected by growth medium, surface type and incubation temperature
Autores: | Jerônimo,Heloísa Maria Ângelo; Queiroga,Rita de Cássia Ramos do Egypto; Costa,Ana Caroliny Vieira da; Barbosa,Isabella de Medeiros; Conceição,Maria Lúcia da; Souza,Evandro Leite de.
Data: | 2012-12-01
Ano: | 2012
Palavras-chave: | Staphylococcus aureus/food processing; Biofilm; Surfaces; Sanitizers/efficacy.
Resumo: | This study assessed the effect of different growth media [BHI broth, BHI broth plus glucose (10 g/100 mL) and BHI broth plus NaCl (5 g/100 mL)] and incubation temperatures (28 or 37 ºC) on the adherence, detachment and biofilm formation on polypropylene and stainless steel surfaces (2 x 2 cm coupons) for a prolonged period (24-72 h) by some strains of Staphylococcus aureus (S3, S28 and S54) from food processing plants. The efficacy of the sanitizers sodium hypochlorite (250 mg/mL) and peracetic acid (30 mg/mL) in reducing the number of viable bacterial cells in a preformed biofilm was also evaluated. S. aureus strains adhered in highest numbers in BHI broth, regardless of the type of surface or incubation temperature. Cell detachment from surfaces revealed high persistence over the incubation period. The number of cells needed for biofilm formation was noted in all experimental systems after 3 days. Peracetic acid and sodium hypochlorite were not efficient in completely removing the cells of S. aureus adhered onto polypropylene and stainless steel surfaces. From these results, the assayed strains revealed high capacities to adhere and form biofilms on polypropylene and stainless steel surfaces under the different growth conditions, and the cells in biofilm matrixes were resistant to total removal when exposed to the sanitizers sodium hypochlorite and peracetic acid.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000400018
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502012000400018
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.48 n.4 2012
Fonte: | info:eu-repo/semantics/openAccess