Data/hora: 02/06/2024 08:25:50

Provedor de dados: 	60	
País: 	Brazil	
Título: 	Validation of analytical methodology for quantification of cefazolin sodium pharmaceutical dosage form by high performance liquid chromatography to be applied for quality control in pharmaceutical industry	
Autores: 	Pedroso,Tahisa Marcela; Salgado,Hérida Regina Nunes.	
Data: 	2014-03-01	
Ano: 	2014	
Palavras-chave: 	Cefazolin sodium/determination; High performance liquid chromatography/reverse-phase/quantitative analysis; Pharmaceutical industry/quality control; Cefalosporine/quality control; Medicines/quality control.	
Resumo: 	A reversed-phase high performance liquid chromatography method was validated for the determination of cefazolin sodium in lyophilized powder for solution for injection to be applied for quality control in pharmaceutical industry. The liquid chromatography method was conducted on a Zorbax Eclipse Plus C18 column (250 x 4.6 mm, 5 μm), maintained at room temperature. The mobile phase consisted of purified water: acetonitrile (60: 40 v/v), adjusted to pH 8 with triethylamine. The flow rate was of 0.5 mL min-1 and effluents were monitored at 270 nm. The retention time for cefazolin sodium was 3.6 min. The method proved to be linear (r2=0.9999) over the concentration range of 30-80 µg mL-1. The selectivity of the method was proven through degradation studies. The method demonstrated satisfactory results for precision, accuracy, limits of detection and quantitation. The robustness of this method was evaluated using the Plackett–Burman fractional factorial experimental design with a matrix of 15 experiments and the statistical treatment proposed by Youden and Steiner. Finally, the proposed method could be also an advantageous option for the analysis of cefazolin sodium, contributing to improve the quality control and to assure the therapeutic efficacy.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000100022	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/S1984-82502011000100022	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.50 n.1 2014	
Fonte: 	info:eu-repo/semantics/openAccess