Data/hora: 10/06/2024 18:58:54

Provedor de dados: | 60
País: | Brazil
Título: | Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells
Autores: | Lima,Melissa Pires de; Hilst,Luciana Farhat; Mattana,Fernanda Vanessa Rechinbach; Santos,Cid Aimbiré de Moraes; Weffort-Santos,Almeriane Maria.
Data: | 2010-06-01
Ano: | 2010
Palavras-chave: | Apocynaceae; Cell proliferation; Himatanthus lancifolius; Indole alkaloids; Leukemia; Plant extract.
Resumo: | The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not undergoing apoptosis or necrosis, suggesting cytostatic activity for tumor cells
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000200014
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502010000200014
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.46 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess