Data/hora: 29/05/2024 03:43:24

Provedor de dados: 	Brazilian Journal of Pharmaceutical Sciences	
País: 	Brazil	
Título: 	Astrocytic expression of GFAP and serum levels of IL-1β and TNF-α in rats treated with different pain relievers	
Autores: 	Amaral,Gisele Ferreira; Dossa,Pietro Domingues; Viebig,Lígia Bocamino; Konno,Fabiana Toshie Camargo; Consoli,Amanda; Martins,Maria de Fátima Monteiro; Viani,Flávio Cesar; Bondan,Eduardo Fernandes.	
Data: 	2016-12-01	
Ano: 	2016	
Palavras-chave: 	Neuropathic pain/treatment; Astrocytes/pain regulation; Analgesics/tests/treatment; Cytokines; Glial Fibrillary Acidic Protein/study.	
Resumo: 	ABSTRACT Pro-inflammatory cytokines and glial cells, especially microglial cells, have been implicated in persistent pain sensitization. Less is known about the role of astrocytes in pain regulation. This study aimed to observe the expression of the astrocytic biomarker glial fibrillary acidic protein (GFAP) and the serum levels of interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) after short-term administration of central pain relievers in rats not submitted to noxious stimuli. Male Wistar rats were divided into five groups, receiving for nine days- (1) amitriptyline (Amt-10 mg/kg/day, by gavage); (2) gabapentin (Gb-60 mg/kg/day, by gavage; (3) methadone (Me-4.5 mg/kg/day, intraperitoneal route [IP]); (4) morphine (Mo-10 mg/kg/day, IP); or (5) 0.9% saline solution, IP. Brain samples were collected for immunohistochemical study of GFAP expression in the mesencephalon and nucleus accumbens (NAc). The area of GFAP-positive cells was calculated using MetaMorph software and serum levels of IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. Serum TNF-α levels were decreased in the groups treated with Mo, Me and Gb, but not in the Amt-treated group. IL-1β decreased only in rats treated with Me. The astrocytic expression of GFAP was decreased in the brainstem with all drugs, while it was increased in the NAc with Amt, Me and Mo.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000400623	
Editor: 	Universidade de São Paulo, Faculdade de Ciências Farmacêuticas	
Relação: 	10.1590/s1984-82502016000400006	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Pharmaceutical Sciences  v.52 n.4 2016	
Fonte: 	info:eu-repo/semantics/openAccess