Data/hora: 13/06/2024 06:56:38

Provedor de dados: | Brazilian Journal of Pharmaceutical Sciences
País: | Brazil
Título: | Evaluation of cytochrome P-450 concentration in Saccharomyces cerevisiae strains
Autores: | Matuo,Míriam Cristina Sakuragui; Kikuchi,Irene Satiko; Pinto,Terezinha de Jesus Andreoli.
Data: | 2010-09-01
Ano: | 2010
Palavras-chave: | Saccharomyces cerevisiae; Cytochrome P-450/obtention; Cytochrome P-450/cultivation conditions; Ethanol; Carbon sources.
Resumo: | Saccharomyces cerevisiae has been widely used in mutagenicity tests due to the presence of a cytochrome P-450 system, capable of metabolizing promutagens to active mutagens. There are a large number of S. cerevisiae strains with varying abilities to produce cytochrome P-450. However, strain selection and ideal cultivation conditions are not well defined. We compared cytochrome P-450 levels in four different S. cerevisiae strains and evaluated the cultivation conditions necessary to obtain the highest levels. The amount of cytochrome P-450 produced by each strain varied, as did the incubation time needed to reach the maximum level. The highest cytochrome P-450 concentrations were found in media containing fermentable sugars. The NCYC 240 strain produced the highest level of cytochrome P-450 when grown in the presence of 20 % (w/v) glucose. The addition of ethanol to the media also increased cytochrome P-450 synthesis in this strain. These results indicate cultivation conditions must be specific and well-established for the strain selected in order to assure high cytochrome P-450 levels and reliable mutagenicity results.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300011
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502010000300011
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.46 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess