Data/hora: 16/06/2024 01:44:05

Provedor de dados: | 60
País: | Brazil
Título: | Simultaneous estimation of rosuvastatin and amlodipine in pharmaceutical formulations using stability indicating HPLC method
Autores: | Ashfaq,Muhammad; Akhtar,Tazeem; Mustafa,Ghulam; Danish,Muhammad; Razzaq,Syed Naeem; Nazar,Muhammad Faizan.
Data: | 2014-09-01
Ano: | 2014
Palavras-chave: | High Performance Liquid Chromatography/quantitative analysis; Rosuvastatin/quantitative determination; Amlodipine/quantitative determination; Pharmaceutical formulations/quantitative analyis; Acetonitrile; UV Detector.
Resumo: | A viable cost-effective and isocratic approach employing C-18 column (250 mm × 4.6 mm, 5 µm) based HPLC has been utilized to separate and estimate the drugs, rosuvastatin, amlodipine and their stress induced degradation products, simultaneously in pharmaceutical formulations. Focused on ICH guideline parameters, the efficient separation of both drugs and their degradation products was achieved by optimizing a 30:70 (v/v) solvent mixture of acetonitrile and 0.1 M ammonium acetate buffer (pH 5) as mobile phase. The flow rate of the mobile phase was 1.5 mL/min and all the detections were carried out at 240 nm using UV detector. The method was linear in the concentration range of 1-200 µg/mL for rosuvastatin with 0.996 coefficient of determination value. For amlodipine, linearity was in the range of 0.5-100 µg/ml with 0.994 coefficient of determination value. Both the drugs along with their degradation products were separated in less than twenty minutes. The results of within-day and between-day precision were varied from 0.72 to 1.81% for rosuvastatin and 0.83 to 1.88% for amlodipine. The results show that this ICH validated method can be employed successfully for the routine as well as stability quantification of both the active ingredients simultaneously in pharmaceutical formulations.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502014000300629
Editor: | Universidade de São Paulo, Faculdade de Ciências Farmacêuticas
Relação: | 10.1590/S1984-82502014000300023
Formato: | text/html
Fonte: | Brazilian Journal of Pharmaceutical Sciences  v.50 n.3 2014
Fonte: | info:eu-repo/semantics/openAccess