Data/hora: 02/06/2024 03:59:17

Provedor de dados: | 61
País: | Brazil
Título: | Cloning and sequence analysis of tomato cpDNA fragments: towards developing homologous chloroplast transformation vectors
Autores: | Berger,Irving Joseph; Carraro,Dirce Maria; Bock,Ralph; Azevedo,Ricardo Antunes; Carrer,Helaine.
Data: | 2005-06-01
Ano: | 2005
Palavras-chave: | Lycopersicon esculentum; Nicotiana tabacum; Chloroplast DNA; Plastid transformation; Plastome; Tobacco; Tomato.
Resumo: | With the view of developing chloroplast transformation vectors based on homologous targeting regions for tomato (Lycopersicon esculentum L.), plastid DNA fragments of tomato cv. IAC-Santa Clara were cloned and analyzed. Isolation and cloning of PstI/SalI chloroplast fragments into the pBlueScript vectors yielded the plasmids pIJB1 and pIJB2 with cpDNA fragments of 8.6 kb and 6.4 kb, respectively. DNA sequencing and computer analyses revealed that the tomato sequences cloned display from 93 to 100 % of identity to the respective fragments in tobacco, which is more pronounced in coding regions. The intergenic spacers are somewhat less conserved suggesting that evolutionary divergences occurred mainly in these putative non-coding regions. The most remarkable difference found is a 437 bp sequence present in tobacco chloroplast genome in the intergenic region of the genes trnE and trnT but completely absent in the tomato chloroplast DNA. Analysis of restriction enzyme digestion patterns revealed several unique restriction sites in the intergenic spacer regions suggesting potential utility of these sequences in species-specific vector construction for tomato chloroplast transformation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200007
Editor: | Brazilian Journal of Plant Physiology
Relação: | 10.1590/S1677-04202005000200007
Formato: | text/html
Fonte: | Brazilian Journal of Plant Physiology  v.17 n.2 2005
Fonte: | info:eu-repo/semantics/openAccess