Data/hora: 08/06/2024 01:02:14

Provedor de dados: 	Brazilian Jounarl of Veterinary	
País: 	Brazil	
Título: 	Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction	
Autores: 	HEINEMANN,Marcos Bryan; GARCIA,José Fernando; NUNES,Cáris Maroni; HIGA,Zenaide Maria Moraes; VASCONCELLOS,Silvio Arruda; Richtzenhain,Leonardo José.	
Data: 	1999-01-01	
Ano: 	1999	
Palavras-chave: 	Leptospirosis; Leptospira; Semen; Polimerase Chain Reaction; Bovine.	
Resumo: 	Due to the high importance of the venereal transmission of bovine leptospirosis, this study aimed to test the ability of PCR to detect Leptospira interrogans serovar hardjo DNA in experimentally contaminated bovine semen. Employing primers directed to the 16S rRNA gene, 10 bacteria/ml of semen could be detected by PCR. Results achieved in this work show that PCR can have a great potential to detect Leptospira spp. in insemination centers.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000100002	
Editor: 	Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo	
Relação: 	10.1590/S1413-95961999000100002	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Veterinary Research and Animal Science  v.36 n.1 1999	
Fonte: 	info:eu-repo/semantics/openAccess