Data/hora: 10/06/2024 01:24:46

Provedor de dados: | 62
País: | Brazil
Título: | Detection of Leptospira spp. from pure cultures and from experimentally contaminated bovine semen by polymerase chain reaction
Autores: | HEINEMANN,Marcos Bryan; GARCIA,José Fernando; NUNES,Cáris Maroni; HIGA,Zenaide Maria Moraes; VASCONCELLOS,Silvio Arruda; Richtzenhain,Leonardo José.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Leptospirosis; Leptospira; Semen; Polimerase Chain Reaction; Bovine.
Resumo: | Due to the high importance of the venereal transmission of bovine leptospirosis, this study aimed to test the ability of PCR to detect Leptospira interrogans serovar hardjo DNA in experimentally contaminated bovine semen. Employing primers directed to the 16S rRNA gene, 10 bacteria/ml of semen could be detected by PCR. Results achieved in this work show that PCR can have a great potential to detect Leptospira spp. in insemination centers.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000100002
Editor: | Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Relação: | 10.1590/S1413-95961999000100002
Formato: | text/html
Fonte: | Brazilian Journal of Veterinary Research and Animal Science  v.36 n.1 1999
Fonte: | info:eu-repo/semantics/openAccess