Data/hora: 10/06/2024 18:05:50

Provedor de dados: | 62
País: | Brazil
Título: | Comparison of five extenders for canine semen freezing
Autores: | SANTOS,Silvia Edelweiss Crusco dos; VANNUCCHI,Camila Infantosi; SATZINGER,Sandra; ASSUMPÇÃO,Mayra Elena Ortiz D’Avila; VISINTIN,José Antonio.
Data: | 1999-01-01
Ano: | 1999
Palavras-chave: | Diluents; Freezing; Semen; Dogs.
Resumo: | Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid nitrogen. Sperm morphology was evaluated before and after freezing, whereas progressive motility (%) and velocity of forward progression (0-5) were appraised in different periods of the freezing process. The extender tris-fructose-citric acid showed the best post thaw progressive motility and velocity of forward progression compared to the others extenders. Semen freezing increased major sperm morphological abnormalities, regardless of the extender.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500004
Editor: | Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
Relação: | 10.1590/S1413-95961999000500004
Formato: | text/html
Fonte: | Brazilian Journal of Veterinary Research and Animal Science  v.36 n.5 1999
Fonte: | info:eu-repo/semantics/openAccess