Data/hora: 09/06/2024 11:56:36

Provedor de dados: | 63
País: | Brazil
Título: | Characterization and selection of Bacillus thuringiensis isolates effective against Sitophilus oryzae
Autores: | Silva,Najara da; Thuler,Ana Maria Guidelli; Abreu,Irlan Leite de; Davolos,Camila Chiaradia; Polanczyk,Ricardo Antonio; Lemos,Manoel Victor Franco.
Data: | 2010-08-01
Ano: | 2010
Palavras-chave: | Coleoptera; Curculionidade; Crystal protein; Entomopathogenic bacterium.
Resumo: | The entomopathogenic bacterium Bacillus thuringiensis is a control agent with toxic and environmental characteristics that allows the control of pest insects according to the Integrate Pest Management (IPM) precepts. In order to find new strains, potentially toxic to Sitophilus oryzae L. 1763 (Coleoptera: Curculinidae), 1.073 strains of B. thuringiensis from parts of Brazil were used. Genetic material was extracted with InstaGene Matrix kit, used for the amplification of sequences in Polymerase chain reaction (PCR), and viewed in 1.5% agarose gel. The gene cry35Ba class was represented by 60 B. thuringiensis isolates (5.6%), which were then subjected to bioassays with S. oryzae larvae. Among the isolates studied, four caused more than 50% mortality in pathogenicity tests, and the isolates 544 and 622 were the most virulent, as determined by CL50 estimates. The four toxic isolates had spherical, bi-pyramidal and cuboid crystals, and a 44-kDa protein was found in sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE), which coded for the product of cry35Ba genes. These data demonstrate the potential of B. thuringiensis for the management of S. oryzae larvae.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400015
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162010000400015
Formato: | text/html
Fonte: | Scientia Agricola  v.67 n.4 2010
Fonte: | info:eu-repo/semantics/openAccess