Data/hora: 09/06/2024 05:21:35

Provedor de dados: | Scientia Agricola
País: | Brazil
Título: | Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
Autores: | Grabicoski,Edilaine Mauricia Gelinski; Jaccoud Filho,David de Souza; Pileggi,Marcos; Henneberg,Luciane; Pierre,Marcelo Luiz Cunha; Vrisman,Cláudio Mauricio; Dabul,Audrei Nisio Gebieluca.
Data: | 2015-02-01
Ano: | 2015
Palavras-chave: | White mold; Dissemination; Soaking seeds; Diagnostic.
Resumo: | Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluated a molecular assay for the detection of S. sclerotiorum in soybean seeds using a species-specific PCR (polymerase chain reaction) primer set and seed soaking (without DNA extraction) for up to 72 h. The PCR products were amplified in all the samples infected with the pathogen, but not in the other samples of plant material or the other seed-borne fungi DNA. The minimum amount of DNA detected was 10 pg, or one artificially infested seed in a 400-seed sample (0.25 % fungal incidence) and one naturally infected seed in a 300-seed sample (0.33 % incidence). The PCR-based assay was rapid (&lt; 9 h), did not require DNA extraction and was very sensitive.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000100069
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/0103-9016-2013-0395
Formato: | text/html
Fonte: | Scientia Agricola  v.72 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess