Data/hora: 12/06/2024 01:06:59

Provedor de dados: | 63
País: | Brazil
Título: | ISOENZYMATIC POLYMORPHISM AND ACTIVITY OF PEROXIDASES OF COMMON BEAN  (Phaseolus vulgaris L.) UNDER SALINE STRESS
Autores: | BROETTO,F.; CASA,A.M.; MALAVOLTA,E.; LOPES,C.R.
Data: | 1997-09-01
Ano: | 1997
Palavras-chave: | Phaseolus vulgaris; Peroxidases; Polymorphism; Salt stress; Phaseolus vulgaris.
Resumo: | One of the uses of the technique of tissue culture for plant breeding is the identification of cell lines tolerant to salt stress.In order to study the biochemical mechanisms involved in the genetic expression to salt tolerance, callus from embryo axis of four bean cultivars (cv. IAC-carioca; cv. IAPAR-14; cv. JALO-EEP558; CV. BAT-93) were grown in Murashige &amp;amp; Skoog (1962) medium, supplemented with NaCl in the concentrations of 0, 20, 40, 60 and 80 mM. After 14 days callus were harvested and analyzed according to their isoenzymatic patterns and peroxidase activities. BAT and IAPAR cultivars showed two common activity zones in the anodic region, with only one specific enzymatic band to each one (the two fastest migration band); it is possible that the two middle anodic zones detected are products of the same enzymatic locus but from different alleles with different eletrophoretic mobilities. Cv. JALO showed two anodic activities in common with cvs IAC and IAPAR with an exclusive anodic zone of slower migration which showed the most intense activity of all cultivars analyzed. This cv. still showed a dimeric heterozygotic catodic zone in all treated samples. Probably this is the same zone which occurs in homozygosis with fixation of the slower allele for all cvs BAT and IAPAR submitted to all treatments. Cv. IAC showed two anodic bands in common with Cv. IAPAR and cv. JALO. It still showed a faster anodic band in common with cv. IAPAR and an exclusive anodic band of slower migration. It is interesting to say that for this cv. IAC resulting from cultivation in NaCl 20 mM did not show activity in the three slower anodic zones. Cv. IAC showed only one dimeric heterozygotic catodic zone in all treatments. This zone is probably composed by two different alleles from the same locus detected in cv. JALO. Samples from cv. IAC treated with 40 and 60 mM showed a more intense enzymatic activity in the catodic zone. Analyses of the peroxidase activity in the crude extract of the different bean cultivars analysed showed different reations to salt concentration in the cultivation procedures as well as a high increasing of peroxidase activity in cv. IAC and JALO.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90161997000200003
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90161997000200003
Formato: | text/html
Fonte: | Scientia Agricola  v.54 n.3 1997
Fonte: | info:eu-repo/semantics/openAccess