Data/hora: 11/06/2024 03:30:18

Provedor de dados: 	Ciência e Agrotecnologia	
País: 	Brazil	
Título: 	In vitro propagation and acclimatization of genipapo accessions	
Autores: 	Sa,Francielen Paola de; Ledo,Ana da Silva; Amorim,Julie Anne Espindola; Silva,Ana Veruska Cruz da; Pasqual,Moacir.	
Data: 	2016-04-01	
Ano: 	2016	
Palavras-chave: 	Genipa americana; In vitro culture; Micropropagation.	
Resumo: 	ABSTRACT Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT(r) may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000200155	
Editor: 	Editora da Universidade Federal de Lavras	
Relação: 	10.1590/1413-70542016402036015	
Formato: 	text/html	
Fonte: 	Ciência e Agrotecnologia  v.40 n.2 2016	
Fonte: 	info:eu-repo/semantics/openAccess