Data/hora: 01/06/2024 05:50:05

Provedor de dados: | 64
País: | Brazil
Título: | Factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum
Autores: | Ishikawa,Francine Hiromi; Barcelos,Quélen de Lima; Souza,Elaine Aparecida de; Dias,Eustáquio Souza.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Protoplast formation; Lytic enzyme; Anthracnose; Osmotic stabilizer; Filamentous fungi.
Resumo: | The present work reports factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum. The usefulness of protoplast isolation is relevant for many different applications and has been principally used in procedures involving genetic manipulation. Osmotic stabilizers, lytic enzymes, incubation time and mycelial age were evaluated in terms of their effects on protoplast yield. The optimal condition for protoplast production included the incubation of young mycelia (48 h) in 0.6 mol l-1 NaCl as the osmotic stabilizer, with 30 mg ml-1 Lysing Enzymes from Trichoderma harzianum for 3 h of incubation. In these conditions protoplasts production was higher than 10(6) protoplatos ml-1 in the digestion mixture, number suitable enough for experiments of transformation in fungi. Sucrose concentrations of 1.2 mol l-1 and 1 mol l-1 were the most suitable osmotic stabilizers for the regeneration after 48 h, with rates of 16.35% and 14.54%, respectively. This study produced an efficient method for protoplast production and reverted them into a typical mycelial morphology using a Colletotrichum lindemuthianum LV115 isolate.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000100009
Editor: | Editora da Universidade Federal de Lavras
Relação: | 10.1590/S1413-70542010000100009
Formato: | text/html
Fonte: | Ciência e Agrotecnologia  v.34 n.1 2010
Fonte: | info:eu-repo/semantics/openAccess