Data/hora: 06/06/2024 01:13:59

Provedor de dados: | 65
País: | Brazil
Título: | Sustainable production with obtaining glucosamine from crab exoskeletons
Autores: | Montiel-Montoya,Jorge; Valdez-Morales,Maribel; Reyes,Cesar; Barrales-Cureño,Hebert Jair.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Callinectes bellicosus; Extraction methods; Glucosamine hydrochloride; HPLC analysis.
Resumo: | ABSTRACT:  Two chemical treatments, five enzymatic (pectinase, lipase, hemicellulase, hemicellulose-cellulase or lipase-pectinase) and one microbiological (Bacillus subtilis) treatment were evaluated to obtain glucosamine hydrochloride (Gluc-HCl) from the chitin obtained from crab (Callinectes bellicosus) exoskeletons. Chemical treatments were referred as Method A (HCl hydrolysis during 75 min at 90°C) and Method B (HCl hydrolysis during 20 min and 14 h of rest). Glucosamine and, in some cases, N-acetyl-D-glucosamine were identified and quantified by HPLC. Treatments with the greater concentrations of Gluc-HCl in descending order were: lipase (94.4 mg/g), microbiological (45.7 mg/g), lipase-pectinase (22.9 mg/g), hemicellulase-cellulase (20.9 mg/g), hemicellulase (15.3 mg/g), pectinase (10.7 mg/g), Chemical A (7.3mg/g) and Chemical B (7.3mg/g). In terms of yield, the best treatments in descending order were: pectinase (94%), microbiological (94%), hemicellulase (92%), lipase (91%), Chemical B (88%), lipase-pectinase (88%), hemicellulase-cellulase (86%) and Chemical A (28.5%). The two most profound treatments were lipase and microbiological, so they are proposed as part of a viable method to produce Gluc-HCl from crab exoskeletons; they are ecofriendly procedures and could add value to the crab´s productive chain.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900752
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20190021
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.9 2019
Fonte: | info:eu-repo/semantics/openAccess