Data/hora: 09/06/2024 04:26:46

Provedor de dados: | Ciência Rural
País: | Brazil
Título: | Vegetative compatibility and molecular characterization of Fusarium graminearum isolates from the State of Paraná, Brazil
Autores: | Busso,Cleverson; Kaneshima,Edilson Nobuyoshi; Franco,Francisco de Assis; Querol,Carmen Boto; Castro-Prado,Marialba Avezum Alves de.
Data: | 2007-12-01
Ano: | 2007
Palavras-chave: | Fusarium graminearum; Genetic variability; RAPD; Vegetative compatibility groups.
Resumo: | Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the clonal origin of F. graminearum isolates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782007000600050
Formato: | text/html
Fonte: | Ciência Rural  v.37 n.6 2007
Fonte: | info:eu-repo/semantics/openAccess