Data/hora: 16/06/2024 19:01:45

Provedor de dados: | 65
País: | Brazil
Título: | Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis)
Autores: | Paulin,Lília Márcia Silva; Samartino,Luis Ernesto; Conde,Sandra Beatriz; Federsoni,Igor Stefan Poppovic; Ferreira,Fernando; Amaku,Marcos; Ferreira Neto,José Soares.
Data: | 2012-09-01
Ano: | 2012
Palavras-chave: | Serological tests; Serodiagnosis; Buffaloes; Brucellosis.
Resumo: | The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also calculated. The best combinations of relative sensitivity (SEr) and relative specificity (SPr) and Kappa were given by C-ELISA (96.9%, 99.1%, and 0.932, respectively) and FPA (92.2%, 97.6 and 0.836, respectively). The C-ELISA and FPA were the most promising confirmatory tests for the serological diagnosis of brucellosis in buffaloes, and for these tests, cut-off values for buffaloes may be the same as those used for bovines.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900017
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782012005000070
Formato: | text/html
Fonte: | Ciência Rural  v.42 n.9 2012
Fonte: | info:eu-repo/semantics/openAccess