Data/hora: 05/06/2024 17:15:03

Provedor de dados: | 65
País: | Brazil
Título: | Molecular detection of protozoa of the Sarcocystidae family in sheep from the State of Rio Grande do Sul, Brazil
Autores: | Portella,Luiza Pires; Cadore,Gustavo Cauduro; Sangioni,Luis Antonio; Alves,Marta Elena Machado; Chemeris,Raiza; Brum,Larissa Picada; Vogel,Fernanda Silveira Flores.
Data: | 2016-09-01
Ano: | 2016
Palavras-chave: | Sarcocystis spp.; Toxoplasma gondii; Neospora caninum; PCR; Diagnosis.
Resumo: | ABSTRACT: Infections caused by protozoa belonging to the Sarcocystidae family have worldwide distribution and are common in ruminants, leading to considerable economic losses. This study evaluates Sarcocystis spp.,  Toxoplasma gondii and Neospora caninum infections in sheep from Southwest region of Rio Grande do Sul, Brazil. Myocardium samples of 80 sheep raised on extensive system were collected. Tissue cysts were detected by direct examination and presence of infective agents was confirmed by PCR. Macroscopic evaluation did not reveal changes, but direct microscopic examination showed cysts in 76.2% (61/80, 95% CI: 66.9 - 85.9) samples, and all cysts were morphologically similar to those caused by  Sarcocystis tenella or Sarcocystis arieticanis. PCR detected Sarcocystis spp. DNA in 21.2% (17/80, CI: 12.3-30.2) of the tested samples and T. gondii DNA in 15% (12/80, CI: 7.2-22.8). Moreover, 6.2% (5/80, CI: 2.1-13.9) samples contained DNA of both protozoan. The presence of  N. caninum nucleic acids was not observed in tested samples. However, all PCR-positive samples (23.7%-19/80, CI: 14.4-33.1) were also positive by direct examination (microscopic cysts). Thus, a high occurrence of microscopic tissue cysts was detected in sheep from southwest region of Rio Grande do Sul State. Although PCR did not show good sensitivity to identify the causative agents of these cysts, it revealed the presence of  Sarcocystis spp. and T. gondii in ovine cardiac muscle samples. This may predispose the contamination of animals and humans by these protozoa.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901613
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20151365
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.9 2016
Fonte: | info:eu-repo/semantics/openAccess