Data/hora: 01/06/2024 02:45:37

Provedor de dados: | Ciência Rural
País: | Brazil
Título: | Comparison of the effects of triamcinolone acetonide or platelet-rich plasma on expression of extracellular matrix-related genes in equine healthy chondrocytes in vitro
Autores: | Palma,Heloisa Einloft; Gallio,Miguel; Silva,Gabriele Biavaschi da; Cantarelli,Camila; Bertolin,Kalyne; Wolkmer,Patrícia; Wergutz,Julien; Krause,Luciana Maria Fontanari; Krause,Alexandre; Antoniazzi,Alfredo Quites; Brass,Karin Erica; Corte,Flavio Desessards De La.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | MMPs; IL-6; Aggrecan; Cartilage; Osteoarthritis.
Resumo: | ABSTRACT:  In healthy cartilage, chondrocytes maintain an expression of collagens and proteoglycans and are sensitive to growth factors and cytokines that either enhance or reduce type II collagen synthesis. In osteoarthritis, pro-inflammatory cytokines, such as IL-6, induce overexpression of metalloproteinases (MMP) and decreasing synthesis of aggrecan. Use of chondroprotectors agents, such as Platelet-Rich Plasma (PRP) and triamcinolone (TA) are alternatives to reduce the progression of joint damage. In this study, we used chondrocytes extracted from metacarpophalangeal joints of healthy horses as the experimental model. Cells were treated in vitro with PRP or TA. No differences were observed between these treatments in comparison to the control group when the expressions of MMP9, MMP13, IL-6 and ACAN genes were evaluated (P&lt;0.05). With these results, we can suggest that the treatments were not deleterious to equine cultured chondrocyte, once they did not stimulate MMPs and IL-6 synthesis or caused changes in ACAN.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000700601
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180262
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.7 2019
Fonte: | info:eu-repo/semantics/openAccess