Data/hora: 09/06/2024 15:57:38

Provedor de dados: | Ciência Rural
País: | Brazil
Título: | Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin
Autores: | Artioli-Coelho,Fabiane Aparecida; Paiva,Renato; Silva,Luciano Coutinho; Barbosa,Sandro; Beijo,Luiz Alberto.
Data: | 2015-08-01
Ano: | 2015
Palavras-chave: | Passiflora gibertii; Native species; Medicinal plant; Secondary compounds.
Resumo: | Brazil is one of the center of origin of passion fruit and has an important natural variability of the genus Passiflora. Several wild species of this genus are resistant to some pests and diseases and many are considered as medicinal. The aim of this research was to induce callus from in vitro Passiflora gibertiileaf explants for quantification of vitamin C and total phenols. Once the appropriate auxin/cytokine balance promotes callus formation and may optimize the production of secondary compounds and vitamins, calli were induced using a half-strength MS medium with a combination of the auxin Picloran (PIC) and the cytokine Kinetin (KIN). The vitamin C and total phenols were quantified by colorimetric methods from calli after different culture periods. The calli induction was strongly dependent of the combination PIC/KIN. It was observed high vitamin C content (94.8mg 100g-1) during the calli induction period in MS+4.14µM PIC+ 0.207µM KIN. Higher PIC/KIN concentrations promoted an increase in the vitamin C content after three subcultures. The higher PIC (8.28µM)/KIN (0.828µM) concentration was the higher was the total phenols production (66mg tannic acid 100g-1of fresh callus) during the calli induction period.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801459
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20120965
Formato: | text/html
Fonte: | Ciência Rural  v.45 n.8 2015
Fonte: | info:eu-repo/semantics/openAccess