Data/hora: 10/06/2024 09:32:18

Provedor de dados: | 65
País: | Brazil
Título: | Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings
Autores: | Macedo,Luciana Riacciardi; Ribeiro,Alexandre Pinto; Conceição,Luciano Fernandes da; Galera,Paula Diniz; Laus,José Luiz.
Data: | 2010-02-01
Ano: | 2010
Palavras-chave: | Corneal repair; Surgery; Cell proliferation; Ki67.
Resumo: | The clinical, histopathological and immunohistochemical features of the cornea were investigated in adult male New Zealand rabbits submitted to lamellar keratoplasty with microfibrillar cellulose membrane. Thirty animals were divided into five groups (n=6) and evaluated up to 60 days after surgery. Clinical examination revealed moderate manifestations of edema, blepharospasm and photophobia on the second day, which became mild or disappeared after the seventh day. This period was characterized clinically by repair of the corneal defect. Histopathological analysis showed the presence of a thin layer of squamous cells covering the damaged area as early as 7th day, accompanied by a mild infiltrate of polymorphonuclear cells. Blood vessels were observed in the epithelium after the 15th day, which had regressed by day 48. Ki67 antibody labeling showed an increase of proliferating cells in the epithelium by the 15th day and in the stroma by day 30. Remodeling and epithelial adhesion were observed during this period. Microfibrillar cellulose membrane (Bionext®) used for lamellar keratoplasty was found to yield good results considering the good integration of the implant.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200019
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782010000200019
Formato: | text/html
Fonte: | Ciência Rural  v.40 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess