Data/hora: 02/06/2024 03:51:34

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Micropropagation of Chapada Diamantina ornamental bromeliad	
Autores: 	Lima,Andressa Priscila Piancó Santos; Brito,Alone Lima; Santana,José Raniere Ferreira de.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	In vitro propagation; Acclimatization; Sincoraea mucugensis; NAA.	
Resumo: 	ABSTRACT: Sincoraea mucugensis (Wand. &amp; A.A. Conc.) LOUZADA &amp; WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30- and 60-day duration) using activated charcoal; finally, these microplants were transferred to a greenhouse for acclimatization, and covered with transparent plastic cups, as a water loss prevention test method. All the data were submitted to the analysis of variance (ANOVA), and the means were subjected to regression analysis or compared using the Tukey test. The findings revealed that the S. mucugensis stem explants raised in the NAA-rich medium (6.42 to 7.43 shoots/explants) showed high multiplication rates; the shoot rooting was done for 30 days using activated charcoal with the medium. Acclimatization, which was performed by directly exposing the microplants to the ex vitro environment, showed 95% survival rate.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000200400	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20180922	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.50 n.2 2020	
Fonte: 	info:eu-repo/semantics/openAccess