Data/hora: 11/06/2024 18:57:47

Provedor de dados: | 65
País: | Brazil
Título: | Comparison of the protocols for obtaining platelet-rich plasma in dogs: a cellular study
Autores: | Vidal Júnior,André William Masseaux; Silva,Ricardo Siqueira da; Marques,Ana Paula Lopes; Souza,Heloísa Justen Moreira de.
Data: | 2020-01-01
Ano: | 2020
Palavras-chave: | Growth factors; Platelet-rich plasma; Cell therapy; Canines.
Resumo: | ABSTRACT: This study aimed to evaluate two protocols (PA and PB) that are used to obtain canine platelet-rich plasma (PRP) for cellularity. Twenty healthy dogs were used. Blood samples were collected and placed in two tubes of 3.2% sodium citrate. PA used double centrifugation at 210 x g, and 370 x g and PB used double centrifugation with 140 x g and 330 x g. The PRP samples from the protocols were examined in terms of their platelet, erythrocyte, and leukocyte count in the Neubauer chamber, differential leukocyte count and platelet morphological observation in blood smears. Data (mean and standard deviation) were analyzed with the 95% probability t-test (P &lt;0.05) using Pearson’s correlation to test the relationship between platelets and erythrocytes, platelets, and leukocytes, and the leukocyte count versus the erythrocytes. Very weak negative correlation between platelets and leukocytes (p= -0.03), weak negative correlation between platelets and erythrocytes (p= -0.3) and a strong positive correlation between leukocytes and erythrocytes (ρ = 0.75) were noted. Although, BP did not reach the desired mean of one million platelets (979300 ± 79631 cells / μL), both protocols, A and B (4.42 ± 1.61 and 3.85 ± 1.55 times more platelets than total blood, respectively) (p &lt;0.05) were efficient in concentrating platelets. Platelet activation was present in 26.55 ± 6.72% of the PA platelets and 26.25 ± 7.03% in PB (p&gt; 0.05). PA and PB presented low erythrocyte concentration (p&gt; 0.05), and PA had more leukocytes (p &lt;0.05) than PB, with higher concentrations of basophils that were segmented, and lymphocytes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300602
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180843
Formato: | text/html
Fonte: | Ciência Rural  v.50 n.3 2020
Fonte: | info:eu-repo/semantics/openAccess