Data/hora: 10/06/2024 16:12:28

Provedor de dados: | 65
País: | Brazil
Título: | In vitro activity of six antiviral drugs against equid alphaherpesvirus type 1 indicates ganciclovir as promising drug for in vivo studies
Autores: | Oliveira,Amanda Lovato de; Cargnelutti,Juliana Felipetto; Mortari,Ana Paula Gnocato; Flores,Eduardo Furtado; Weiblen,Rudi.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | EHV-1; Plaque assay; Acyclovir; Ganciclovir; Foscarnet; Famciclovir; Vidarabine; Cidofovir.
Resumo: | ABSTRACT:  Equid alphaherpesvirus type 1 (EHV-1) is distributed worldwide and is a major agent of abortion, respiratory and neurological disease in horses. No specific treatment is available for EHV-1 infection, yet the potential of antiviral therapy has been explored. In this study we investigated the in vitro activity of Acyclovir, Ganciclovir, Foscarnet, Famciclovir, Vidarabina and Cidofovir against EHV-1. For this, the MTT test was performed, in which all the tested drugs showed no toxicity up to 200μg/mL. Subsequently, different drug concentrations were submitted to viral plaque reduction assays in cell culture. The selectivity index (SI) of the compounds was determined using the cytotoxic concentration for 50% of cells (CC50), obtained by MTT, and effective drug concentration to inhibit by 50% the number of viral plaques (EC50). Ganciclovir (SI: 490; EC50: 1.9 μg/mL) was the most efficient and safest drug against EHV-1, followed by Cidofovir (SI: 150, EC50: 5.7μg/mL), Acyclovir (SI: 37.4, EC50: 22.2μg/mL), Famciclovir (SI: 25.1, EC50: 24.5μg/mL), Vidarabine (SI: 12.2, EC50: 40.9μg/mL) and Foscarnet (SI: 6.9, EC50: 49.5 μg/mL), respectively. These results indicated that Ganciclovir (followed by Cidofovir), is a promising candidate for use in in vivo experiments.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200450
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180085
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.12 2018
Fonte: | info:eu-repo/semantics/openAccess