Data/hora: 04/06/2024 01:19:26

Provedor de dados: 	65	
País: 	Brazil	
Título: 	Bovine Vaccinia in dairy cattle and suspicion of vesicular disease on milkers in Brazil	
Autores: 	Silva,Thaís Garcia da; Lima,Michele dos Santos; Castro,Alessandra Marnie Martins Gomes de; Martins,Maira de Souza Nunes; Castiglioni,Vivian Cardoso; Fava,Claudia Del; Okuda,Liria Hiromi; Pituco,Edviges Maristela.	
Data: 	2018-01-01	
Ano: 	2018	
Palavras-chave: 	Orthopoxvirus; Poxviridae; Quantitative PCR; Risk factor; VACV; Zoonosis.	
Resumo: 	ABSTRACT: Bovine vaccinia (BV) is a vesicular disease induced by the Vaccinia virus (VACV) that affects milk production and is an occupational zoonosis. This research had the following objectives: (i) detection of VACV by qPCR in cattle with clinical suspicion of vesicular disease; (ii) symptoms characterization in animals and milkers with clinical suspicion of the disease and virus detection in humans; and (iii) identification of risk factors for infections of VACV in herds from several Brazilian states. A total of 471 bovine epithelial samples from dairy farms, in 15 Brazilian states, were evaluated between 2007 and 2012. The samples were tested by quantitative PCR (qPCR) using SYBR Green® reagents, validated with a lower limit of detection of 100 TCID50/50µL (1.7x100 viral particles), and 45.1% of VACV positive samples were detected. Using official forms for epidemiological investigation (FORM-IN), the risk factors for VACV infections in cattle were determined to be farms with a lack of technological facilities (P=0.029) and the presence of rodents (P=0.001). There was an effect of seasonality in cattle with a higher occurrence of BV during the dry season. A total of 420 epidemiological questionnaires were applied at public health care centers, where 100% of the milkers had vesicular lesions on their hands (98.1%) and on their arms (6.9%). The most frequent clinical symptoms in humans were: local swelling (74.2%), headache (20.7%), fever (10.4%) and inguinal lymphadenopathy (74.2%). Only 19.98% of milkers aged between 39 and 58 years were seroreactive to VACV and were immunized with the human anti-smallpox vaccine. There was an increase in the frequency of BV in older individuals due to their natural decrease in specific immunity. It has been shown that the implementation of zootechnical management techniques and health planning are important for the prevention of BV in animals and humans.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500451	
Editor: 	Universidade Federal de Santa Maria	
Relação: 	10.1590/0103-8478cr20170723	
Formato: 	text/html	
Fonte: 	Ciência Rural  v.48 n.5 2018	
Fonte: 	info:eu-repo/semantics/openAccess