Data/hora: 11/06/2024 10:10:28

Provedor de dados: | 65
País: | Brazil
Título: | Protein and antigen profiles of Leptospira interrogans serovar Hardjo
Autores: | Lafetá,Bárbara Nobre; Castro,Elaine Cristina de; Silva,Nivaldo da.
Data: | 2009-12-01
Ano: | 2009
Palavras-chave: | Leptospira interrogans; Outer membrane protein; Hardjoprajitno; Immunomodulatory proteins.
Resumo: | The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera from naturally infected bovines.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782009000900024
Formato: | text/html
Fonte: | Ciência Rural  v.39 n.9 2009
Fonte: | info:eu-repo/semantics/openAccess