Data/hora: 08/06/2024 16:05:26

Provedor de dados: | 65
País: | Brazil
Título: | Micropropagation of two species of foliage anthurium by direct organogenesis
Autores: | Campos,Arlene Santisteban; Carvalho,Ana Cristina Portugal Pinto de; Castro,Ana Cecília Ribeiro de; Bertini,Cândida Hermínia Campos de Magalhães.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Anthurium bonplandii; Anthurium maricense; 6-BA; Tissue culture.
Resumo: | ABSTRACT: The objective of the present research was to develop a protocol for micropropagation of Anthurium bonplandii and Anthurium maricense by direct organogenesis. Nodal segments, with two or three nodes, were used as explants. The cultures were kept in a growth chamber at a temperature of 25±2ºC, under a photoperiod of 16 hours and a luminosity of 30μmol m-2 s-1. At 60 days, the number of regenerated buds per explant (NBE) was counted. The experiment was carried out in an entirely randomised design consisting of six treatments for six different concentrations of 6-benzylaminopurine (6-BA) added to the P2 (Pierik) medium (0.0, 1.11, 2.22, 3.33, 4.44, and 5.55µM). All the treatments were performed in four repetitions with 10 culture tubes containing one explant each. The regression analyses were adjusted to a quadratic model, with R2 = 88.7% and 62.4% for A. maricense and A. bonplandii, respectively. The regressions indicate that the addition of 6-BA to the P2 medium resulted in larger values of NBE in both the species. The ideal 6-BA concentration for micropropagation varied depending on the species, with 2.5 and 1.7 NBE determined at 6-BA concentrations of 4.70 and 3.37µM for A. maricense and A. bonplandii, respectively.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700401
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170817
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.7 2018
Fonte: | info:eu-repo/semantics/openAccess