Data/hora: 16/06/2024 09:17:24

Provedor de dados: | 65
País: | Brazil
Título: | Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis
Autores: | Silveira,Marcelo Marques da; Cândido,Stéfhano Luis; Santos,Karin Rinaldi dos; Maia,Maerle Oliveira; Souza,Roberto Lopes de; Sousa,Valéria Régia Franco; Almeida,Arleana do Bom Parto Ferreira de; Dutra,Valeria; Nakazato,Luciano.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Blood culture; PCR; Bacteremia; Fungemia.
Resumo: | ABSTRACT:  Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600452
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170871
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.6 2018
Fonte: | info:eu-repo/semantics/openAccess