Data/hora: 09/06/2024 06:44:25

Provedor de dados: | 65
País: | Brazil
Título: | Exposure of Baixadeiro horses to Rickettsia spp. and to ticks infected by Rickettsia amblyommatis in the Baixada Maranhense micro-region, Maranhão, Brazil
Autores: | Amorim Filho,Edvaldo Franco; Costa,Francisco Borges; Moraes-Filho,Jonas; Santos,Ana Clara Gomes dos; Vale,Tássia Lopes do; Costa,Andréa Pereira da; Silva,Arannadia Barbosa; Labruna,Marcelo Bahia; Nogueira,Rita de Maria Seabra.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Baixadeiro horse; Ticks; Rickettsia spp.; Maranhão.
Resumo: | ABSTRACT:  The aim of this study was to investigate exposure of Baixadeiro horses to Rickettsia spp. and to ticks infected by Rickettsia in the Baixada Maranhense (lowlands) micro-region, state of Maranhão. A total of 258 horses were tested for Rickettsia rickettsii, Rickettsia amblyommatis and Rickettsia bellii using the immunofluorescence assay (IFA). Overall, 58.91% (152/258) of the horses were seroreactive for at least one Rickettsia species, and 85.27% of the horses were infested with one or more species of tick, which were identified as Dermacentor nitens (93.63%), Amblyomma cajennense sensu stricto (4.55%) and Rhipicephalus (Boophilus) microplus (1.82%). These ticks were subjected to DNA extraction and were tested using the polymerase chain reaction (PCR), targeting two rickettsia genes: citrate synthase gene (gltA) and 190kDa outer membrane protein gene (ompA). Three specimens of A. cajennense s.s. were positive. BLAST analyses on the nucleotide sequences obtained from the PCR products showed that these were 99-100% identical to the corresponding sequences of R. amblyommatis. Thus, results indicate that R. amblyommatis and/or a strain very close to this is circulating in ticks in this micro-region.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900453
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180002
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.9 2018
Fonte: | info:eu-repo/semantics/openAccess