Data/hora: 09/06/2024 04:30:06

Provedor de dados: | 65
País: | Brazil
Título: | Genetic variability in physic nuts cultivated in Northeastern Brazil
Autores: | Oliveira,Vanice Dias; Rabbani,Allivia Rouse Carregosa; Silva,Ana Veruska Cruz da; Lédo,Ana da Silva.
Data: | 2013-06-01
Ano: | 2013
Palavras-chave: | Jatropha curcas L.; RAPD; Genetic diversity.
Resumo: | This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and formed by the most divergent individuals. AMOVA indicated that there is more variation within (63%) the population. In conclusion, it was possible verify genetic variability in physic nut using RAPD markers at these experimental areas.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600006
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782013005000060
Formato: | text/html
Fonte: | Ciência Rural  v.43 n.6 2013
Fonte: | info:eu-repo/semantics/openAccess