Data/hora: 11/06/2024 11:04:03

Provedor de dados: | 65
País: | Brazil
Título: | Induction and identification of polyploids in Cattleya intermedia Lindl. (orchidaceae) by in vitro techniques
Autores: | Silva,Paulo Artur Konzen Xavier de Mello e; Callegari-Jacques,Sídia; Bodanese-Zanettini,Maria Helena.
Data: | 2000-03-01
Ano: | 2000
Palavras-chave: | Cattleya; Colchicine; Chromosome number; Orchid improvement; Polyploidy; Stomata analysis.
Resumo: | The effect of colchicine on the protocorm-like bodies of Cattleya intermedia L. (Orchidaceae) cultured in vitro was studied. Different concentrations of the drug (0.00, 0.05, 0.10 and 0.20%) as well as two times of culture (4 or 8 days) were tested to determine the best treatment for the induction of tetraploid plants. Of the 3 colchicine levels applied, 0.05 and 0.10% of drug treatments appeared to be effective on the production of mixoploids and tetraploids and could be further used in breeding programs. The suitability of stomatal area and stomata density as criteria for the distinction between diploids and tetraploids was also tested. The results suggested that tetraploid plants could be identified with a fair amount of certainty when the screening was based on the density of stomata.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000100017
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782000000100017
Formato: | text/html
Fonte: | Ciência Rural  v.30 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess