Data/hora: 02/06/2024 14:50:09

Provedor de dados: | 65
País: | Brazil
Título: | Molecular characterization of virulence genes cctA, nanA, and fliC in Clostridium chauvoei from Rio Grande do Sul and São Paulo State, Brazil
Autores: | Ziech,Rosangela Estel; Farias,Luana D’Avila; Ardisson-Araújo,Daniel Mendes Pereira; Camargo,Gregório Miguel Ferreira de; Frey,Joachim; Vargas,Agueda Castagna de.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Blackleg; Clostridium chauvoei toxin A; Neuraminidase; Flagellin.
Resumo: | ABSTRACT:  Clostridium chauvoei toxin A (CctA), neuraminidase (NanA), and flagellin (FliC) proteins contribute to the pathogenicity of Clostridium chauvoei, the causative agent of blackleg in cattle. The aim of this study was to analyze the genetic variability of cctA, nanA, and fliC genes in C. chauvoei isolates from the Rio Grande do Sul and São Paulo state- Brazil, during different sampling periods. The presence of these genes was verified through PCR amplification and partial gene sequencing of 17 strains. Alignment of PCR amplicons combined with bioinformatics analysis was used in an attempt to study the variability across C. chauvoei solates. The similarity among the partial sequences of cctA and nanA genes was 100%. The sequencing of fliC revealed three different paralog alleles of flagellin, and two strains were seen to be polymorphic, with amino acid alterations in the predicted protein. Overall, this study indicates that strains of C. chauvoei isolated in Brazil are highly conserved with respect to the virulence factors evaluated.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500452
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20181006
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.5 2019
Fonte: | info:eu-repo/semantics/openAccess