Data/hora: 12/06/2024 14:50:37

Provedor de dados: | 65
País: | Brazil
Título: | Molecular detection of Trypanosoma evansi (Kinetoplastida: Trypanosomatidae) in procyonids (Carnivora: Procyonidae) in Eastern Amazon, Brazil
Autores: | Magalhães-Matos,Paulo Cesar; Cunha-Santos,Rafaelle; Sousa,Paulo Geovani Silva; Sampaio-Júnior,Francisco Dantas; Barros,Flávia de Nazaré Leite; Mourão,Fábio Rodrigo Paixão; Oliveira,Wanessa Batista Lima; Gabriel,Áurea Martins; Monteiro,Silvia Gonzalez; Góes-Cavalcante,Gustavo; Scofield,Alessandra.
Data: | 2016-04-01
Ano: | 2016
Palavras-chave: | Trypanosoma evansi; PCR; Procyonidae; Eastern Amazon.
Resumo: | ABSTRACT: The present study aimed to diagnose the natural infection of captive and free-living procyonids with Trypanosoma evansi in the states of Amapá and Pará, Brazil. From February 2012 to August 2013, whole blood samples and blood smears were obtained from 45 free-living procyonids and from nine procyonids kept in captivity in wild life refuges and zoobotanical parks in the states of Amapá and Pará. Whole blood samples were collected and kept at -20ºC for the detection of T. evansi DNA by PCR using the RoTat 1.2 forward and RoTat 1.2 reverse primers. In addition, the blood smears were processed and examined for the presence of trypomastigote forms of T. evansi. T. evansi DNA was detected in 18.52% (10/54) of the procyonids, namely, in captive crab-eating raccoons and captive and free-living coatis in Pará State. No trypomastigote forms were observed in the blood smears. DNA from T. evansi was detected in P. cancrivorus and N. nasua in Pará State, being this the first such report in P. cancrivorus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400663
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150679
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess