Data/hora: 09/06/2024 15:05:56

Provedor de dados: | 65
País: | Brazil
Título: | Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples
Autores: | Makino,Herica; Sousa,Valéria Régia Franco; Fujimori,Mahyumi; Rodrigues,Juliana Yuki; Dias,Alvaro Felipe Lima Ruy; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de.
Data: | 2016-02-01
Ano: | 2016
Palavras-chave: | Canine ehrlichiosis; Diagnosis; Nested PCR; Biological samples.
Resumo: | ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20150090
Formato: | text/html
Fonte: | Ciência Rural  v.46 n.2 2016
Fonte: | info:eu-repo/semantics/openAccess