Data/hora: 08/06/2024 10:22:53

Provedor de dados: | 65
País: | Brazil
Título: | Production and characterization of alginate microparticles obtained by ionic gelation and electrostatic adsorption of concentrated soy protein
Autores: | Silverio,Gabriela Barros; Sakanaka,Lyssa Setsuko; Alvim,Izabela Dutra; Shirai,Marianne Ayumi; Grosso,Carlos Raimundo Ferreira.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Microencapsulation; Ionic gelation; Electrostatic interaction; Alginate; Soy protein.
Resumo: | ABSTRACT:  Microencapsulation is used for protection and release of bioactive compounds. Combination of encapsulation methods allows the production of matrices with better technological properties compared to the application of one of the methods alone. Use of ionic gelation produces porous microparticles, and coating it with a protein, by electrostatic interaction, may contribute to a better protection of the active compound. The objective of the research was to produce alginate microparticles (AG) through ionic gelation and to coat them with soluble protein from soy protein concentrate. Two factors were studied, calcium concentration during ionic gelation (0.8, 1.6 and 2.4% w/w) and pH (3.5 and 7.0) of the protein solution for electrostatic interaction. Zeta potential (ZP) of biopolymers and microparticles were determined. Microparticles were characterized according to its morphology, average size and size distribution, as well as protein adsorption. Microparticles presented (154-334μm) multinuclear distribution of active compound, continuous and smooth surface, with a great standard deviation considering average size. The calcium concentration did not influence the protein adsorption on microparticles.The pH used in protein adsorption showed significant effect, with higher adsorption occurring at pH 3.5 (6.5 to 6.7% w/w, dry basis,db, of adsorbed protein) compared to pH 7.0 (&lt;2.0% w/w, db, of adsorbed protein) indicating that electrostatic interaction was determinant for the protein coating. At this situation, ionic gelation microparticles and proteins presented ZP with opposite charges (pH&gt;pKa AG&lt;Isoelectric point, IP).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001200750
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180637
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.12 2018
Fonte: | info:eu-repo/semantics/openAccess