Data/hora: 09/06/2024 13:27:32

Provedor de dados: | Ciência Rural
País: | Brazil
Título: | Phytochemical screening and antimicrobial activity testing of crude hydroalcoholic extract from leaves of Sphagneticola trilobata (Asteraceae)
Autores: | Leite,Ana Greice Borba; Farias,Eulina Tereza Nery; Oliveira,Ana Paula de; Abreu,Ruan Emmanuell Franco de; Costa,Mateus Matiuzzi da; Almeida,Jackson Roberto Guedes da Silva; Estevão,Lígia Reis de Moura; Evêncio-Neto,Joaquim.
Data: | 2019-01-01
Ano: | 2019
Palavras-chave: | Antimicrobial; Phytochemical Screening; Phenolic compounds.
Resumo: | ABSTRACT:  This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were identified through phytochemical screening using analytical thin-layer chromatography. Antimicrobial activity was evaluated by testing cultures of Staphylococcus aureus, S. epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, and Klebsiella pneumoniae isolated from human skin and those of Staphylococcus spp. isolated from dog skin using the broth microdilution method. In the phytochemical screening, classes of anthracenic derivatives and mono-, sesqui-, and diterpenes were identified. Colorimetric analysis showed total phenol and total flavonoid contents of 21.7 ± 0.009 mg of gallic acid equivalents per gram of sample and 0.23 ± 0.005 mg of catechin equivalents per gram of sample, respectively. Microbiological analysis revealed that the hydroalcoholic extract of S. trilobata exhibited antimicrobial activity against cultures of Staphylococcus spp., E. coli, S. marcescens, and E. faecalis isolated from human skin and those of Staphylococcus spp. isolated from dog skin. Thus, crude hydroalcoholic extract of leaves of S. trilobata contained flavonoids and terpenoids as secondary metabolites, which contributed to its antimicrobial activity against skin bacteria isolated from different sources.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000400452
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20180639
Formato: | text/html
Fonte: | Ciência Rural  v.49 n.4 2019
Fonte: | info:eu-repo/semantics/openAccess